FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1794_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252596
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT6200.038.5964551
TATACTG32400.031.7810675
GCGAAAG20600.028.5130042
TTACGTG8550.027.9880732
AGCGAAA20900.027.7567631
GTAATAT27700.027.4874631
GTTGACA36500.026.224441
CGAAGCG1409.988564E-425.8980545
GTATTAT61750.024.3085211
ACATGGT38850.023.8915375
CGAAAGC26400.022.5234583
CCGACTA9350.022.4911444
TACAGCC40200.020.9204127
TAGACCG1750.003656520320.7184415
CTCCGAC5103.274181E-1119.901407145
ATACTGT54900.019.812726
GTGATCG7400.019.5947979
AGTCGAA4102.166962E-819.4551222
GTAGCAT57950.019.3956091
ACCGTAC5657.2759576E-1219.251651
Produced by FastQC (version 0.11.5)