FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1794_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922258
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG46820.11937001594489706No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT44230.11276667674589484No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA40610.10353729917817747No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT39620.10101324288203377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCG553.2203703E-452.726585145
TCGCCAT13350.042.90206145
GTTACGT4950.038.085651
TTACGTG4950.038.0851672
GTTGACA28150.031.9401191
ATTCGAC2755.456968E-1231.6403881
TATAGCG2807.2759576E-1231.0749862
TATACTG21350.030.5655615
ACATGGT31050.028.4895735
GTAGCAT59800.028.3731751
TAGCATG54150.027.8517572
TACGTGT6900.027.3219683
GCGAAAG12100.026.9658972
CTACTGA39900.026.7139266
GTATTAT56550.026.4136161
GGATAGC10550.024.7421685
GCATGTT61650.024.4634654
AGCATGT62050.024.0720543
GTATAGG13950.023.9098531
CGCTATA4251.8189894E-1223.8850862
Produced by FastQC (version 0.11.5)