FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1794_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7493418
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC416720.5561147129387417No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA242540.3236707200906182No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG225550.30099748872944226No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC212180.28315516363827564No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA175650.23440571445500574No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG167310.2232759469710618No Hit
GGTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCT152630.20368542099212938No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT147180.19641237149722596No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC143780.19187505621600182No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC136920.1827203553838849No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT133860.17863677163078318No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT127620.1703094635852424No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG122720.16377039156230175No Hit
TGGTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCC122690.16373035642746742No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT117140.15632385648311625No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA114410.15268065921319218No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC108940.14538091962839922No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC106410.14200462325737068No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG105170.14034983768421833No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG104680.13969593048192427No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC102810.137200407077251No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT100250.1337840755713881No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG97450.1300474629868506No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG95750.12777880534623853No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA94970.12673789184054593No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA93900.1253099720314548No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT91730.12241409727843822No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG91410.12198705584020536No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT90250.12043903062661125No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC89570.11953156757036643No Hit
TGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATTG87990.11742305046909167No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC86580.11554139913187812No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC85670.11432700004190344No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT81190.10834841990664341No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT81020.10812155414258219No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC80600.10756106225490157No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA77670.10365096408608195No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT76640.10227642445676992No Hit
CCCTTAGGTGACTCTCCAATTGGCCATGTCTCCGATCTGTTTTCAAAGAA76530.10212962896237739No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT75320.10051487852405938No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA21300.0104.1676562
GGTACTG20750.0102.038615
GACTTAA1950.081.7934145
AGTAGAA24100.080.933131
AACACTG45350.077.386975
AGGTACT29400.074.482894
GTACTGA33150.068.9016656
GCGAAAG9500.062.58662
CAGGTAC35500.059.8453453
CCTCGCG902.0521838E-856.387882145
ATATCAC11350.053.0241053
AGAAACA38100.051.1946074
ATCACTA11900.051.183755
CCCACGC2700.051.017605145
GGCGACG1004.7095455E-850.74909145
GGATAGC52400.049.539495
TCCAACA73650.049.0283972
ACGTAAG604.9470994E-448.333448
GTCGCCC752.5524543E-548.332466145
TCCGCGG752.5524543E-548.332466145
Produced by FastQC (version 0.11.5)