FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1794_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTTCATAGTCCTGCCTGGAAGGCTTTATGGCCACGACCTCTGTTTT11.25No Hit
TCCCCACACAGCTTCTAACAGTTGTAGAGAAGTGGAATTACGGGCTCTCT11.25No Hit
GGTCGCCACCTCCTATGAGTGTATTAAGTATATTTGCATTGGCGAATACT11.25No Hit
GGGTGAACTTGAGCTTCTGCACCTCCTCAAAGTGGTAGTCGAGGACGATC11.25No Hit
GGTCTATATATTCCATCAGATTTTACTTACTAGAGTGATGTTAAATTCTT11.25No Hit
GTGCGGCTGCGGCGTGGGTCGGATGCTGGTGGAGCATTCGCTCTCTATGG11.25No Hit
GGTTTAGTCGTGTCCGTTAGTTATAGTTAGATCACACTTCTTTTGGAACC11.25No Hit
TGTGTGGGGAGAACCTGGGAGAAGGTTGTCCTTCTATCCTCTGTCCCTCA11.25No Hit
GCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGGATTAGAAATC11.25No Hit
ATAATAATGCTGATCGCCAAGGGTGATGCGAGGATTCAAGGAGCACCTGG11.25No Hit
GTATTTCATTTATTTAAGTTCTTATGTGTTCCTTCTACTAAATTTGTAAT11.25No Hit
GTTGTTAAGTGGGCAGCATATGGATCCGCTGTTTATCGATCCTGATGTGT11.25No Hit
TCTCCAATAAGCAATCTAACTTGATACCTCAAGGAAACAGAAAAGAACAA11.25No Hit
CTCAAGTGATTAGGAATTTTAATAAAACAAGACTTTAGCTACGTCTCTGC11.25No Hit
CAATGTGACATCGTGACTGGCTCTCGAAGAATGAGTCACCTCTGGGCATC11.25No Hit
CCAGCTGGGCCCCCGTGGCCCAGCTGTCGGGGGGTCGGCGGCAGGTTGGC11.25No Hit
TGCTAGGACCACCGTTCCCTTCTTCCAGGGCCCCAAGTACTCCCCAACCC11.25No Hit
TCCCCCTCTAATGCATCTTTCTTCCCCCTCTAATGCATTCAGTGCAGCCA11.25No Hit
GAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAAC11.25No Hit
CGGCAGGGGCGGCTTATTCCCCCACATACGCCAGATTCAGCAACGGATAC11.25No Hit
CCTTGGGAGGTTTCGATAAGGGCCATGCTGGCCTCCTAAAATGAGGTGGG11.25No Hit
GGGTATGGCGGTTCGCCATTTAAAGTGGTACGAGAGCTGGGTTTAGAACG11.25No Hit
GGGTGATAGTTTTTGAAATGGTCAAGAGTTGACCATCCAAGAATAAAAGG11.25No Hit
GGGCTTAGTCTGTTGTATCCGGATAGATCGCTTGAAACGTCAATGAAACA11.25No Hit
GTCACGCTGGCCTTTTTGCGTTGGGGAAAACCTGACGGGTGCGCAGCTGG11.25No Hit
GTGCGCAGCGATGCGGCGGACCTCTATCGCCAGCAGGATCTTAACGTCCC11.25No Hit
GGGCGGCTGCGGCGTGGGTCGGATGCTGGTGGAGCATGCGCTCGCGCTGG11.25No Hit
GGCCTGGAGTCTGATGTGAGGGGTGACATCTGTCTTGGAAGGAGGCGGGA11.25No Hit
CTCGGTCTCTGTGTGTCGGGAATCTAGAAGTGTACGGGTTTGAGTCATCT11.25No Hit
TGTTCAGACTGCTACAGATTAAATCAGCACGCAGCAGCGGGCTGAGAAAA11.25No Hit
GTGTTCAGACTGACTCAGGGCACAGTTTGTCTACTCTAAGTACACGGCCT11.25No Hit
GGAGGAAGGAAGGCTTGGGCTAAAGTTGGCCGCTCTTAGATGCCTGTGTG11.25No Hit
AGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGAC11.25No Hit
AGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTGGCGCAAAAA11.25No Hit
GCGAAGGCAGACCCTTAACGACTGAGACACGCAGGCACCCCCAGTATTTT11.25No Hit
CCGCACCACCGTCCAGCTGCCCAAGGTGCCATTTTCAGAGTAAGCAGCTT11.25No Hit
CTCCTGTGTCAGCCGCTCACAGGGCACTGCCTGGGGTAGAAGACAACCTC11.25No Hit
CGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGA11.25No Hit
TATCGGGCTCCTGTGGATTCGTCTCCACCTGAACTCCTAATCCCCACTTT11.25No Hit
GTCAATTAACCTCTCTGAAACTCTGTTTCCATATCTCTAAAGTTATGATA11.25No Hit
TCTCTATTCAATAGGTTAAGAATCGACAAAACCAGGCACAGCCCATACAA11.25No Hit
CTATTGCTCCAAAGTTTCTTCATCTTTATTCTGATTCATCCTTTGGTTGT11.25No Hit
TAGGTACTGCGCTCTTAGAGCAGACCAGGATTTCCCATGAATATGAATGC11.25No Hit
GTACATTAGTAAGTATACAGTAAGGAGTTTTGGGGGGTGGTTGTGGGGAT11.25No Hit
GTTTCAATCTTCTTCGGCATCAGGTTAAGGATCAGAACCTTAAGTGGACG11.25No Hit
TAACAATGTTATGGACGATATATGCCATAAGCTTAACTTTTTACCCCTGC11.25No Hit
GGCAGTTCCGCAACATGATCAAGTGCACGATCCCTGGGAGTGAACCCTTG11.25No Hit
GTGCCCCCTCTGCGGCGCCAGCGTGAGGCCCGAGGCCAACAAACAAGAGC11.25No Hit
TCTCAAGAGTTCAGAGGAGCAACGATCACTGTTTTCTGAGGCAATTGGGG11.25No Hit
GGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAAGCCTAGAT11.25No Hit
ACCTTATAGTAATAGCCCGTCATCCAGCGCACGCGCCAGTTATCTTCGAC11.25No Hit
CCCCATGCGAGAGTAGGGACCTGCCACGCATCAAATTAAGCGAAAGGCCA11.25No Hit
GCCCTGGCTTACCCACAGGGAGCCACAGAGATCTGTCATGAAGCTTCCTG11.25No Hit
TTCCTGGCAGGTTGGTAGAGCTGTAGTAGAGCTAGTAAGTGGCTGTGCAA11.25No Hit
GGCCAGGCCTGGGGAGGAGAGTTGTGCTTGGGCCAGCTCCCCGGGTCCGC11.25No Hit
GTGGGTAAGTGTGGCCTGCAATTGTGGGGGGCCCAAAGATTGGTGAGTTT11.25No Hit
TCTCTCTCCCCAGCTCTGTGTCAAATAAATAAATGGCATCTTTAAAAAAA11.25No Hit
CGTTTAGACCTCCATATAGCTGAAGAAAACCAGCAAATTTGCAAATGTCG11.25No Hit
CGCCTGCGCCAGCCACTTCCTCCCTCCAGGACACCCAATTTCTGTAAATC11.25No Hit
CTATAGACGTACTGAATGAAAGATCTTGAGAGCTTCGTGCATTGGTGAAC11.25No Hit
GTCCATATTGAGAATTTGAATCCATTAATATGGAATATTTTCCCACTTAT11.25No Hit
ACCGGGGACAATGATCATTTTTAGCCCCAGTTCCCGGGCGCGCTCACCTT11.25No Hit
GATGTCTGCCTTCTGCCAGGATGGCTCACCCTCCCCAGATCAGGCTAGGT11.25No Hit
CATCACCTGCACGAATAGCTTCCCGATTGGCAATAACGCTCAGCCCGTTA11.25No Hit
GTGGGGGATTAAGCCGCCCCTGCCGTGCATGCCCAGGCGGGCGAAGACGC11.25No Hit
CCGTGAGACACAGGGCTGAAGCGCCCAGACTCTTGGAATTCTGGGGAAAT11.25No Hit
GTGTCAGCGAAGCGAGGCGTGGAGCTACCCGGTACTAATGAACCGTGAGG11.25No Hit
ACATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCTGGAGACT11.25No Hit
ACGATCCACTCAAAAGCCAAACGAGTTAAAAAAATAAAAAAAAAAAAAAC11.25No Hit
ATCCTGGGAAAAGGGCTCAGGGAAAAAAAAAAAAAAAACAAAAAAAACCC11.25No Hit
GGAAGCTGCAGACGGATGGGAATGGTGTTTCCAGCTACTGTACAAATGAC11.25No Hit
GTATCAGGCTGAAAATCTTTCTCTCATCCGCCAAAACACCTTCGGCGTTG11.25No Hit
GGCTGGAGAAGACGCGCCAGCGTCGCACCCGGCATTTTTTCACCAACGCA11.25No Hit
AACCAGGAGAGTTGGTGTTCTGCGAACCATGTATTTTATAGGAGGAGTTA11.25No Hit
CGGCATGAGGAAGGGGAGGAACTCGTTGCGATTTGGTGTCGATCTGTCGA11.25No Hit
GAACCACCATTGCATCCCTGAAATACTGCTTACTGGATCATACGTCTTGA11.25No Hit
GCCGGCTCAAGATTAAATCAGACCGAAGCAGCGGGCGGCTAACACATCAG11.25No Hit
GTGTGTTCACATGTGTGTACCTGTGTGTGTGTCTGTGTGTCTGTGTGGGT11.25No Hit
GATACAGGCCTAACTCAAGAAACAAGAAACGTCTCAAATAAGCAACCTAA11.25No Hit
GGTCTCTTTTCACACAATGGCAGTCATGCTGCTTAGATGGTGAGTTCTAA11.25No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCA50.0145.05
CGCCTGC50.0145.01
CCAGCCA50.0145.09
CCTGCGC50.0145.03
CGCCAGC50.0145.07
GCCAGCC50.0145.08
CTGCGCC50.0145.04
GCGCCAG50.0145.06
AGATTAA50.0145.0145
GCCTGCG50.0145.02