FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1794_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7109815
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC710260.998985205662876No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG638730.8983778058922771No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG608450.8557887933792931No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA378560.5324470467937633No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG339170.4770447613615825No Hit
GTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA308130.4333868040167009No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT299530.42129084933996175No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC238320.3351985951814499No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA213340.300064066364596No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA205470.2889948613290219No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC199640.280794929263279No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA190350.26772848519968523No Hit
CACTTAGAAGTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAA184470.2594582278160543No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG183630.25827676247553555No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA177700.24993617977401664No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG168300.2367150200110692No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG164090.23079362824489807No Hit
TTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACA160880.2262787428364873No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA155910.21928840623841828No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT154500.2173052322739762No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA146380.20588440064896202No Hit
GTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGGTGTTCAACAGCC144400.2030995180605965No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC142070.1998223582470149No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA141160.19854243746145295No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA137230.1930148674754547No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT131320.18470241490109096No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG124300.1748287402696132No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT121520.17091865259503938No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG119940.168696372549778No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG119450.16800718443447546No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG118210.16626311655085257No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT115660.16267652533856367No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT114320.16079180681916477No Hit
GAATATAGGCTGTTGAACACCGTCAATGAAATGGAAGTCTGAATACATGA113500.15963847160580127No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG109950.154645374035752No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG109430.1539139907297166No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA107660.15142447447648075No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT107120.15066496104329016No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT106590.14991951267367717No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGA106180.1493428450669954No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC100900.14191649149802069No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA98730.1388643727016807No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC98400.1384002256036198No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC97180.13668428784715214No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT96580.1358403840324959No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG95940.13494021996352928No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG95510.13433542222969233No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG94870.13343525816072568No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC93860.13201468673938774No Hit
ACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG92960.13074883101740342No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGGTGT92890.13065037557236017No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT90910.12786549298399466No Hit
GCAATATGCACACACTTAGAAGTCTATATTCATCTCCACAACTCGAAGGA90870.12780923272968425No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC90680.12754199652170978No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT88850.12496808988700832No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTC88520.1245039427889474No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA87740.12340686782989431No Hit
GTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATGAA87190.1226332893331261No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT87160.12259109414239329No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG86230.12128304322967616No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT86060.1210439371488569No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA85310.11998905738053661No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG85080.11966556091825174No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA84100.1182871846876466No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC83580.1175558013816112No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA83530.11748547606372317No Hit
AATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGA83130.11692287352061902No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA80700.11350506307126135No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA80140.11271741951091555No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC78190.10997473211328283No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG77150.10851196550121206No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC76640.10779464725875426No Hit
GTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCG76450.1075274110507798No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT75400.10605057937513142No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA75250.10583960342146737No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA74940.10540358645056164No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT73870.10389862464775808No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG73340.10315317627814506No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT72690.10223894714560085No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG72320.1017185397932295No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC71880.10109967699581494No Hit
GTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT71800.10098715648719411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA94700.0114.087452
GGTACTG101150.0106.527225
AGTAGAA46450.0102.4046551
CAGGTAC107300.0100.622843
GTACTGA116800.092.936586
GACTTAA1700.089.55681145
AGCAGGT141650.076.2218861
GCGAAAG12150.072.212212
AGGTACT155800.071.533764
TTTTTGG148650.065.9011
TTTGGAC149750.065.707443
ACTGATC180950.064.5462958
AGAAACA74350.064.465174
GACAGTA155250.063.324937
CGAAAGC14350.060.636053
GGACAGT166550.059.6463176
CTGATCC182550.059.5329259
AACACTG26700.058.66115
TTGGACA180950.057.904714
CTCCGAC4400.056.02147145
Produced by FastQC (version 0.11.5)