FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1794_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7298418
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG180110.24677950755903538No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC175060.23986019984056817No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG165360.22656964838133417No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT157920.2163756583961072No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC147290.2018108581887198No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT138220.18938350749436386No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG135620.18582109163931143No Hit
TTTCTAAACCATTCAGGTTGGTTCCTTGTGATGTATGTTATCATTGCTAG98650.13516627850035445No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA93460.12805514838969212No Hit
CTGTAACTCCAATGCTCATGTCAGCCGATTCATTAATTCCGGAAACTCCA87110.1193546327436987No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA86740.11884767356432585No Hit
CCATAGAGGTATTCAGATCGAACGGTCTAACAGCCAATGAGTCAGGAAGG86560.11860104477436069No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA86210.11812148879387287No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG85990.11782005360613766No Hit
TTATAGGGGCAATGCTCAAGACATTTCTAAACCATTCAGGTTGGTTCCTT82910.11359996097784479No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG82860.11353145298063225No Hit
GTCTTAGGAGTCTCAATCTTAAATCTTGGGCAGAAGAGGTACACCAAAAC82400.11290117940627681No Hit
CTGTAAAGGAGAGCTCTGTGTCTTGTGAGTTAGTCATCATCTTTCTCACA79830.1093798683495519No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA76730.10513237252237403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA32550.084.42562
GGTACTG34600.078.37575
GTACTGA42650.065.962736
CAGGTAC41950.065.8535163
AGGTACT49100.058.0359344
ACTGATC59800.052.737258
TTTGGAC45100.049.6784323
TATAGCG48500.047.0928272
GCGAAAG18350.047.021542
CGAAAGC19150.044.299933
GACAGTA50400.043.874347
TCACGCG855.345655E-542.645924145
GGATAGC60050.042.3818665
GCGGTAT55350.041.1337176
TACTGAT70750.040.0674447
TTGGACA65950.039.6897474
GTATAGG59400.038.6953471
AGTAGAA47300.037.5570031
GTATCCC62150.037.0957689
CGGTATC62800.036.4811677
Produced by FastQC (version 0.11.5)