FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1794_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4211362
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC79690.18922619333127857No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG78050.1853319662380009No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG71470.169707567290582No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG65800.1562439894741891No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG64400.15291964927261062No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG62890.14933411091233667No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG49900.11848898289911909No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA47090.11181655720880798No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC46680.11084300043548857No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA45990.10920457562185344No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT43530.10336323498193697No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC43430.10312578211039564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG11500.054.2264822
GGTACTG20250.047.9827845
GCAGGTA21700.044.4429362
CGAAAGC14750.042.278273
TATAGCG9950.040.810862
AGGTACT27350.038.9731644
GACAGTA28750.038.33147
GGATAGC34000.036.895455
GCGGTAT11100.036.5822756
TTTGGAC31850.036.4268263
TATACTA9700.035.8823052
AGTAGAA34650.034.3207361
GTACTGA33250.034.2384156
GTATAGG39550.033.5521661
TTGGACA37000.032.728074
TCGCCAT29900.032.006355145
TTTAAAC37300.031.8820173
CAGGTAC31200.031.3754523
ACTGATC40050.031.1353848
CGCTATA9800.030.3367182
Produced by FastQC (version 0.11.5)