FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1794_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12128
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC230.1896437994722955No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG180.1484168865435356No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG160.13192612137203166No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG160.13192612137203166No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG150.12368073878627969No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA100.0070051365145.02
GAGTACG100.0070051365145.0145
CTAGTCT100.0070051365145.09
CCATACC100.0070051365145.04
GGAGGGC100.0070051365145.03
ACTAGTC100.0070051365145.08
TCCATAC259.1626326E-487.03
TTTTTAC300.001889693772.52
ACCCTGT451.1470096E-464.444447
TTACCCT400.005907993354.3755
GTTTTTA400.005907993354.3751
TTTTACC400.005907993354.3753
TACCCTG400.005907993354.3756
CCCTGTT604.7526768E-448.3333328
TTTACCC450.00941234248.3333324
GGGGGGG3650.00116364399.931506145
Produced by FastQC (version 0.11.5)