FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1793_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1793_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971433
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT30770.10355273028198851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG10300.040.8316962
CGAAAGC12300.033.602873
AGCGAAA11800.031.9542031
GCACCGT22950.027.8048956
AGCACCG23800.025.8973875
TATACTG16100.025.6721945
ACCGTGT24400.024.959188
TAACCGT1600.00217372922.6605936
CTATACG1600.002174154222.6598324
CACCGTG30400.022.1794227
ATAGCAC31600.021.3403993
CCGTGTT27450.021.129829
TAGCACC31300.020.8499414
CTTAGAC11600.020.628263
TTAGACT18700.019.7758524
ACATAGC32600.018.9063631
CAATCCG3902.7353417E-718.590189
CATAGCA34850.018.3099232
GTGATCG5651.4370016E-1017.9650239
CTAATAC17650.017.6656823
Produced by FastQC (version 0.11.5)