FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1793_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1793_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294476
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG79500.15015650273983677No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC76940.14532127447550997No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA66480.12556483398923707No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC61910.11693319603299741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG8050.034.224839
TATACTG30550.033.7019775
GTTGACA44000.033.617071
ACATGGT51300.028.4090655
ACTCGCG3351.8189894E-1228.134012145
GCGAAAG22350.026.926432
TAGCATG81600.026.923452
GTAATAT28700.026.2744581
AGCGAAA23050.026.1089551
GGTCCTA72250.025.5891539
GCATGTT87300.025.331674
GACATGG59750.024.998134
GTATTAT61100.024.56471
AGCATGT94000.023.3718453
TGACATG66750.023.24563
CGAAAGC26400.022.5210253
GTCTACG4256.184564E-1122.1787341
ATGTTGT110750.022.06326
TTACGTG4659.094947E-1221.8299792
GCAGTGC45100.021.5432171
Produced by FastQC (version 0.11.5)