FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1793_d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1793_d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTAACCAACTGAGTCACCTGGGCCTCCTGCATTTGCTTGATTTTCT13.0303030303030303No Hit
GGCCAGGGGCATCCCCTTTGTTCTTGAAGCAAGAAACATGGCTGAGGTCT13.0303030303030303No Hit
AAGATGTCTTTATCAAGACAGGAGGGCAGTTCACCTTCTTGGGCCTCCCT13.0303030303030303No Hit
TATCTGGATTGCCAGTTCAGGAAGGAGGGAATGGATCACATAGCACCGTG13.0303030303030303No Hit
CCCCGATTTGGCTGGCCAGACCCCAGTCGTGGTGACAAGAATAATCGAGC13.0303030303030303No Hit
CCAATACCATAGATATAACAATAACACTTAGGTCCGTTTACAAGTGAGAC13.0303030303030303No Hit
GTAATATACTGTGCTGCGTCTGAGAAACCCGCAGGGCCTGTTAATTCTTT13.0303030303030303No Hit
GTGTGTGGGAGAGAGAGAGAGAGAGAGAGAGTGAGAGAGAGAGATAGAGA13.0303030303030303No Hit
GGCGCAGTGGGGGGGCCGCGATGTGGGTGAGCAGTGGTCTGGGTGGGGTG13.0303030303030303No Hit
TTGGTCATGGCTGCAGAACTAACAGATGATCTTATTTTCAGTTCCAATGG13.0303030303030303No Hit
GACACACGGACAGCCTGAGCAAATTGGACGGCGCCGCCGGCAGTAGAAGA13.0303030303030303No Hit
CACTAAATAATTCTGGAATAAATGAAAGCTTTGAAGTCATTTAGGCTAGC13.0303030303030303No Hit
GAGTTGTAGCATTCACTGGTGAAACCCGGGGGTCCTGGCCTTTTGCTTGT13.0303030303030303No Hit
GCGGCCCATTAGTTTGATTTGTGGATGTAGAACCCCCATTGCATCCCTGA13.0303030303030303No Hit
GTGGTTTAACAACACTATAATTATGCTATGCTCATTACAAGTATAGTTCC13.0303030303030303No Hit
AGGTAGGGCTGAGAAAGGAGGGCTGCATTTGGAATAATCATTCCCAGCAG13.0303030303030303No Hit
GCTCATGGTGCGGATGAAGGCTGGTTGTGGGCCTGAGTTTGGGTCTGGGC13.0303030303030303No Hit
CGTCGGTTTCCTTGGCCATGGAGCTGGAAAACGCATGGGTGCCCCTTACT13.0303030303030303No Hit
GAGGGAGACCGAGTGGGCGGCCCTGGGACATCCCCAGCTCGCCCCTGTGC13.0303030303030303No Hit
GTCTGTCAGTTTGTCTCTCTCTCCAGCTCTGTGTCAAATAAATAAATGGC13.0303030303030303No Hit
AGGTGACACACCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGCTGGA13.0303030303030303No Hit
CATTAAATGAAAGACTCATGAGCTCTTTCCAGTTTCTGACATTCAAACAT13.0303030303030303No Hit
GAATTATGTTTACAAACTAAATAAGGTAGTGCATGGGCTCTGAAGATACA13.0303030303030303No Hit
TGGGACACGTGGGTGGCTCAGTTGGTTGGTTAAGCAGCTGCCTTCAGCTC13.0303030303030303No Hit
ACACATAACCCACCACGCCACCCAGGCCTCCCTTAGTGCCAGCGTTAAAA13.0303030303030303No Hit
GTCGATGATTAACATGTTTATGAACCCCGGAGAGTTGTGGGCCGTTATAT13.0303030303030303No Hit
AAATTAGCCAGAATTTATGAAAAGAGTTTGCATATCCCTGTGTAAAGTAT13.0303030303030303No Hit
GGTATGGTTGGTACCATGTGCTGGGAGGCAGGCGGGGGGCGCCGGGGGCG13.0303030303030303No Hit
GGCTGAGGCTGCGCCGGGGGTGGGGTCTCCTGTTGGCTGCGTTTTAGTCC13.0303030303030303No Hit
GCCCTGAGAAGACCTTTCATCCTCACCCCTGACAGATCCAGAATATGCAA13.0303030303030303No Hit
GCGTAGGGATGGCCTGCCCTTTTGCTCTGGCGTTCCGGTGGACAGGGGAC13.0303030303030303No Hit
GGGTTAAGATGAGTTGCTTAAACATATTTTTATTATTTCTTGAATACACT13.0303030303030303No Hit
ATGGCTGAGTTATGTCTAGAAATACAGCCCTGAAATGAAATCTTTGTGGG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)