FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1793_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1793_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5172859
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA23350.035.4022831
GCGAAAG25050.033.2892042
CGAAAGC29400.027.8704763
CAATTCG3706.666596E-921.5538319
TATACTG32800.020.1178155
GTCCTAT38150.019.0072271
GCACCGT29200.017.879816
TAGTACT17950.016.9667284
AGCACCG30650.016.7973655
GTATATA30650.016.7973651
ACCGTGC7401.8189894E-1216.6550718
TATACTA11350.016.6108075
ATTATAC24500.016.57433
GTATAAG14350.015.6647361
TCTATAC53150.015.41659453
ATGCCGG12800.015.292662145
ACGTCCG6651.4515535E-915.2639
CCTAGAC16800.015.1067863
CACCGTG39550.015.0320317
ACCGTGT34800.014.5830418
Produced by FastQC (version 0.11.5)