FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1793_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1793_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGAGGAAGGCTGGAGGCAGGAGGCTGGGCCGCGGCCGCGCCTCCTGA15.88235294117647No Hit
AGACCACCCTCCCCAGAGGGAGCGTGCAAATACTGAAGCAAAAAAGTCAA15.88235294117647No Hit
GGAGTTTTTAATTTTTATATTACATCGTGATGAAAAAATAATGGACAGAG15.88235294117647No Hit
CCCTTGCCCCTAGTATATGAACTCAGGGTTCTCCAATCGAGCCCAGCATT15.88235294117647No Hit
CCTTACGAAAGAGATTTCAATGGCATCTGTAAGAAATCATGGACATTGAG15.88235294117647No Hit
CCCAGGGACAGCGTCGAAGGACCTCTATATTCCCATTACAGCATTATACA15.88235294117647No Hit
GTCTCGATCCTGTTGCTGTCCAGGTAGAGCTGGTGAATGGCCGTGGGCAC15.88235294117647No Hit
ATGTTATACCATGTGAAATGTGGATTGTCTCCTTGTGTGTCTTTTAAAGG15.88235294117647No Hit
TCCACAGGGAGAAGAGAAGTTTATACAGTTTTAAGTGTCTTGAAGGAGAT15.88235294117647No Hit
AGCCATCTCCAAGCCAAAAAAAATGTCTCACAAGACATAAATCCTGCACC15.88235294117647No Hit
CAGGACCCCAGGTTCATGACCTGAGCTGAAGGTAGATGCTTAACCCACTG15.88235294117647No Hit
GGATGCATACACCGTAGATTATTCCGCATCTAATCCATCCGTCTTCGAAC15.88235294117647No Hit
CATATATCGGGTGCTGGGCACCTAGTTCAAGGAATGAGTTATCACATGTG15.88235294117647No Hit
CCTTGGAAACACTGACCAGTGCCCTGGTAACACAAGAACTTGCCCAGCTG15.88235294117647No Hit
TCCCAGCACCCTGGGACCATGACCTCAGCTGAAGCCAGATGCTAAACAAA15.88235294117647No Hit
CTACAACTGATAATGGTATTAATGAAGTAAAATGATGCAACATTTATAAA15.88235294117647No Hit
CATTAGGTATGCTTCCTCCAGAGGGGATTTACTTTTGTTTCTTAGAGTAG15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)