FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1793_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1793_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4329401
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG7000.036.249539
TATACTG26000.032.3505945
TATAGCG5300.028.7300262
GCGAAAG16900.028.317172
CGAAAGC17050.027.642773
TAATCGT2859.91713E-925.438279
CGCTATA5750.025.2205622
GCGTATA1751.2555983E-424.8602661
GTAATAT24800.023.6824111
AGCGAAA20100.023.448221
GCGGTAT6900.023.1191146
GTATAGG17250.023.1188471
TCGCCAT19150.021.200525145
GTACCGA12300.020.6300537
TTAGAGT17900.019.4437834
GGATAGC13650.019.1235035
TACCGAC13850.018.8445228
GTTACGT4253.2745447E-818.7670651
ACTGTGC40200.018.575638
GTCTACG3953.1244963E-718.3567371
Produced by FastQC (version 0.11.5)