FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1793_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1793_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909058
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT47730.12210102792028156No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC42840.10959162028294285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG2250.041.88827145
TATAGCG2300.040.9844742
GTATAGG19200.035.8782961
GCGAAAG17500.032.7335362
GTACGAC2003.2541175E-729.0043963
AGCGAAA19200.028.7026351
TATACTA12150.027.4530745
TATACTG28900.027.3487855
GTTACGT3750.027.0711151
CGAAAGC21250.026.6157973
GTCGGTA9850.025.025013145
TATAGGT27200.024.5257762
CGCTATA5050.024.409642
TTACGTG4002.7284841E-1123.5660722
TACACCG5900.023.3512955
GCACCGT33250.022.2442596
AGCACCG34500.021.8586645
GCGGTAT4701.0913936E-1121.5992956
TATACCG14200.021.4471875
TAATCGT2404.3649692E-521.1471449
Produced by FastQC (version 0.11.5)