FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1793_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1793_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158197
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC85590.16593007207751082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG20900.040.588882
AGCGAAA21350.039.0541731
TATAGCG8900.035.8451272
CGAAAGC29250.029.2498463
GGTACTG27350.028.3660015
TCTATAC56050.027.4238053
TATACTG30750.027.3517155
ACTGATC38450.027.3409658
GTACTGA31900.026.820266
TATACCA67750.025.7916685
GCGGTAT12600.025.3194246
GACAGTA34150.025.2640327
CAGGTAC30350.025.0840723
CGTACGG1450.001226580925.0019385
AGCGGTA13350.024.4400985
AGGTACT32950.024.4250244
TAGCGGT14550.024.4174654
CGGTATC13000.023.9812367
CTATACC67150.023.7544044
ACGTACG1550.0018087923.3886834
Produced by FastQC (version 0.11.5)