FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1790_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1790_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683476
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC40060.10875596854710062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG18950.030.6103972
AGCGAAA19800.028.9301131
CGAAAGC22650.025.9301513
TATACTG23750.025.6454185
CCTTACG5051.8189894E-1221.5371442
TATAACG3259.833493E-720.0792452
TACACCG6700.019.4801275
ATACCGC5407.4578566E-1118.7987256
GTATTAT30400.018.365611
GTATAAG14500.018.002081
TATACGG6152.5465852E-1117.6852115
CTCCGAC4557.1575414E-817.52712145
CTTACGG5802.0736479E-1017.5020243
GTATTAG13300.017.4456251
ATAGGAC25700.017.2101613
GTCCTAT35850.017.1916681
GTCTACG7800.016.7327041
GTATATA32950.016.724241
ATATACG4801.315475E-716.6165074
CGCGGCC7051.0913936E-1116.45357145
Produced by FastQC (version 0.11.5)