FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1790_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1790_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4960483
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG21750.032.0032232
AGCGAAA23700.028.1462941
CGAAAGC27500.025.3118933
GTCTATG30200.022.3284071
TACGTCG4607.2759576E-1222.0656597
GTATAAG9000.019.335281
GTATAAT25450.019.0883661
TATACTA14100.019.026935
TAGGACT45350.019.0263634
TATAATA43150.018.6519362
TCTATAC47850.018.6384283
ATTATAC20800.018.4756033
TCTATGA40350.018.3289812
TATACTG23550.018.1654955
TAGTGCA37050.018.0034229
ACGACGA2851.6344112E-417.8073738
GTCCTAT36250.017.801791
CTATGAC28150.017.7728393
GTAGGAC38850.017.7304083
TACACTG35050.017.3771045
Produced by FastQC (version 0.11.5)