FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1790_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1790_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4398901
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG19400.038.1233752
AGCGAAA20600.034.494651
CGAAAGC24000.030.8163973
TATACTG25800.025.575235
TATAACG6150.022.401465
TATACCG3157.2837065E-720.717145
GTCCTAT32750.020.3689881
ATAATAC49950.019.3067463
GTGATCG7150.019.265769
TAATACA53600.018.9391774
TATATCG3101.46688435E-518.7122555
ATACAGC43800.018.7069156
TATAATA53400.018.4667552
ACGTCCG8950.017.8212559
CGACGTT2500.00139440217.4000249
ATGCCGG10950.017.214245145
ATAACGT10100.016.5121776
CTAATAC20100.016.2333973
TACCGTG3304.9989804E-415.3789847
TCCTATG38900.015.2847082
Produced by FastQC (version 0.11.5)