FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1790_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1790_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4648147
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG20000.041.6926572
AGCGAAA22050.037.487631
CGAAAGC26200.031.5497043
TATACGG6300.026.4715295
TATACTG36200.025.2379285
CTAGTAC11250.023.202873
TACGGGG8800.023.0683067
ATATACG7250.023.0028464
GTACTAG9050.020.8313051
TAGTACT17750.020.016564
TATACCG2205.90289E-419.7751734
TTACGGA7450.019.4654944
GTCCTAT43550.018.314551
TCTATAC43200.018.1272413
CCTTACG8650.017.6033342
GTTCTAT53900.017.3537241
TACGGAG8900.017.1088585
GTATAAG14050.017.0305751
GTCTACG3052.741899E-416.6414031
TAATACA66100.016.3446854
Produced by FastQC (version 0.11.5)