FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1790_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1790_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229496
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG700.001056212641.432747
TCTATAC7600.038.1617363
GCGAAAG7350.036.500272
GTTCTAT9000.033.0311051
AGCGAAA7700.032.9578631
CGAAAGC8850.030.3137873
TATACCA10600.029.4145375
CTATACC10200.029.1450924
TTCTATA11200.025.8954622
GTATATA2951.4269972E-824.5787471
CTCCGAC2401.5988771E-624.166151145
GTGATCG1851.8297497E-423.5158829
ATACCAC12400.022.8065726
GTGTAGG7350.022.6893581
TATACTA1650.002599061221.9728055
ATGCCGG4307.094059E-1121.918137145
TGCGCAC4351.9645086E-919.999575145
ACTGTCG2959.560188E-619.6621998
CACGAGC5601.2551027E-1018.124613145
GTAGGAC9800.017.756893
Produced by FastQC (version 0.11.5)