FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1790_d04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1790_d04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTACATTTCCAGAAACATACAAACTACTAAGATAGAATCCTAAAGAAA14.545454545454546No Hit
GTCCCTGGGCCAGTGCAAAGATAGGAGCAATGAATTCCCAATCACAGAAG14.545454545454546No Hit
GAATGACTAGTAACCACTTTTATCTCAGTAAAGCAGCTATTTGTCCAAAC14.545454545454546No Hit
CACTGACTACATCCTGTGTACCTTGGCGTCACCAGTAATAACCACAGTCT14.545454545454546No Hit
GCATATCAGTGGTTTCAGGGAACATCTCTCTCATTTCTGATGATGAGTCC14.545454545454546No Hit
CTTGTAATATACTGTGCTGCGTCTGAGAAACCAGCAGGGCCTCTTAATTC14.545454545454546No Hit
TAGTGATACAGGCCTAACTAAACAAACAAGAATCGTCTCAAATAATCACA14.545454545454546No Hit
GCCATATAGAAAAGCACTTAGGGAAAATCATTGTGTGACTAACTGAATGA14.545454545454546No Hit
GAGAATATGTATAAAAGTGCTTTATAAAGTAGGAGATACTGCCCAATTAT14.545454545454546No Hit
TCCGGATACCGCCCCCCAAATATGTTCAAACGCCCCATTTGGCCTATGGC14.545454545454546No Hit
ATGTGGGTCAGTGAAGGAGCGGAACAAAAATTGACACGGGGACAGGAGCC14.545454545454546No Hit
ACCCTGGGATCATGACCAGAGCTGAAGCCAGGTGCTAAACAAACTAAGCC14.545454545454546No Hit
GAGAGAAAGGAGTGAAGCAAAGAAAAGGAAGCGAGCAACGGAAGAGAAAT14.545454545454546No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCCACTTTAAAA14.545454545454546No Hit
GCTTATAAACAGCACAAATATCCTTCTCACAGTTCTGGTGACTGGAAGTC14.545454545454546No Hit
TGAGACATTCCATGTAAACACCTCAGTAAGCAACAGTGAGCCACTGTCCC14.545454545454546No Hit
GTATAAAAGTGCTTTATACAATAGCAGATTCTGCCCAATTATTAAAATGA14.545454545454546No Hit
GCATATATGAACAAACCTAAGAGAAGCAATCAGCACCAATATCATAGGGA14.545454545454546No Hit
CCTAAATTCATGACTTGAGCCAAAGGCCAACACATAACACACCAAGCCCC14.545454545454546No Hit
GCCCTGCCCCAGGGGCATATGCATACACCTGTGAATACACAGAGAGAGAG14.545454545454546No Hit
GGGTCTACTAAAACAAGGATCATACACAGAAAAGCTGATGGCAGCAAGGT14.545454545454546No Hit
CATCCCCCTCAGCCACCCCCTTACTTCGATCGACATGACAGCAACACACA14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)