FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1790_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1790_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809822
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG18600.051.4547652
AGCGAAA19700.048.2144741
CGAAAGC20350.046.3173333
CGTAGAC1102.4363905E-432.9565623
TCTATAC21750.032.3353163
GATATAG6050.031.1594871
GTAATAT12550.029.4644811
GCGACTT1504.40127E-529.0002298
TGATCCG8000.028.0939719
CGAATAC1855.822307E-627.431671145
GATGCGC7450.027.2485378
TATACTG23500.027.1510835
CTATACC19100.026.1927034
TACTCGG5550.026.1281915
ATGCCCG2859.915311E-925.437891145
TATACCA21300.025.1897855
CCGGTAA3301.7771526E-924.165997145
ATCGATA3352.1136657E-923.8061588
ATATACT20400.021.6802484
GCCCGTA3055.346865E-721.3936129
Produced by FastQC (version 0.11.5)