FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1789_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1789_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335229
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT29900.12803883473526578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG7900.033.9603922
GTGATCG4450.032.5845879
AGCGAAA9100.030.278911
TAATCGT1952.6096131E-729.7438819
TATACCG2603.7198333E-927.8884725
GTTACGT2156.123082E-726.9804741
GTCGGTA2507.8411176E-826.099695145
ACTACGC2301.1028424E-625.2208771
ACGACTC2601.1445809E-725.0996255
CGAAAGC11150.024.7119353
CCTATAC7800.024.1700063
TATACTG13350.023.8984345
TACGGTG1851.8299703E-423.5167645
GCACCGT19950.022.89796
CGCACAG3854.274625E-1022.6005275
GTACCGA2903.2889693E-722.50077
CTACGGT2003.1018557E-421.7530064
GTTGACA16900.021.452671
CGCCGCA3055.3393705E-721.39642
ATACCGT2755.2093474E-621.0938226
Produced by FastQC (version 0.11.5)