FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1789_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1789_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305138
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG2800.038.8447722
TATACTG24450.026.6911985
GCGAAAG18350.026.0799772
GTGATCG7800.025.09629
CGAAAGC22150.021.6057593
AGCGAAA21250.021.4974021
CTATGCG7400.019.5973634
CGCTATA2256.866198E-419.3360632
GTAATAT22900.018.9985371
GTATATA23100.018.5201451
GTATTAT32600.018.461411
TATACCG4755.858965E-918.318595
CCCTACG7300.017.8765378
TAGTACT15250.017.5926484
GTATAAC15700.017.5504531
ATACTGT37950.017.3873756
CCTACGC7451.8189894E-1216.5436559
ATAGCGG5703.105015E-916.5374243
TAATATA28650.016.450842
GTATAGG15500.016.373471
Produced by FastQC (version 0.11.5)