FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1789_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1789_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6559604
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT162330.24746920698261662No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC118690.18094080069467608No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT79150.1206627717160975No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC77640.11836080348752759No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC74600.11372637738497628No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG74370.11337574646274379No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA68640.10464046305234279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC8950.037.266951
TTACGTG7050.035.9971352
GTTACGT7250.033.0038761
CTACGCA10600.030.7819182
TATACTG39300.030.2580075
TAATCGT3800.028.6193839
AGCGAAA27050.027.073421
GCGAAAG29100.026.1628652
GTAATAT35650.024.2034021
CGAAAGC33450.022.110223
GTGATCG8000.021.750739
GTCTACG3706.657501E-921.5565851
GGATTAG40100.021.5174881
AGCACCG113750.021.41795
TCGCACC5450.021.2869034
GCACCGT114100.021.1618796
ATTAGGT37950.021.016963
GTTGACA35550.021.0080951
ATCTAAA96000.020.9972571
CACCGTG123950.020.5893277
Produced by FastQC (version 0.11.5)