FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1789_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1789_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916805
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT117830.3008319280638173No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC93000.23743842238763482No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT63000.1608453829077526No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT60030.1532626719992443No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC56950.14539911994597637No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA56910.14529699589333653No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC56330.1438161971300588No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC51850.13237830323439642No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG51710.13202086905015695No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC50710.12946776773416088No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA48590.1240551929442492No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT43320.11060034900894988No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC42720.10906848821935226No Hit
GTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTACAAGCTGTAATCA40470.10332401025836108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG3750.052.208282
GTTACGT3900.050.2002751
TTACCGC2400.039.2770651
GTAATAT18050.036.958771
GTTGACA22850.036.8110921
GTAGAAA28550.035.0493432
TATACTG35150.034.863735
ACTACGC6700.034.632361
GGTCGTT8900.033.3994837
GACGACT3700.033.316524
CAGTATG14350.032.840329
ACGTGTT6200.032.7475474
ACATGGT24650.032.358495
AGCGAAA15150.032.067791
GACAGTA15750.031.3021567
AAGGTCG9550.031.130995
AGACGAC4300.030.354043
TACGTGT6950.029.213643
TTAGGTA18700.029.0825254
CTACGCA8400.028.4866622
Produced by FastQC (version 0.11.5)