FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1789_d06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1789_d06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23268
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC340.1461234313219873No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC320.13752793536187038No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT300.12893243940175347No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG250.10744369950146124No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT250.10744369950146124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGCA100.007003252145.299563
TATTGCT100.007048571144.987099
CCTGGGG300.001896322872.649793
AAAAAAA5506.663203E-55.00864580-84
Produced by FastQC (version 0.11.5)