FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1789_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1789_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5287580
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG537371.016287224023088No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG376860.712726805079072No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT317200.5998963609061234No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG292110.5524455421951063No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT231480.4377806104115683No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT220900.4177714568857587No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA217560.4114547675874407No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC208950.39517132601303434No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA204010.3858286777694144No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC188170.35587168421092447No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA187240.35411284557396766No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT180610.3415740281943725No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC174660.3303212433665306No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT162460.3072483064086028No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA154550.292288721872766No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA151770.28703111820530375No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG150280.2842131939374912No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG148040.2799768514140684No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT147720.27937165962500804No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA145420.2750218436411364No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA135100.25550440844393846No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC127860.24181194421644686No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT122440.23156150828923627No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT120450.2277979718510169No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG119720.2264173780822229No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA118000.22316447221602323No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG113820.2152591544714217No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG112400.21257361590746618No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC110760.20947200798853163No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT110580.20913158760718512No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG108930.20601106744484246No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC108290.20480068386672162No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA107850.2039685451567636No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG107540.2033822656111113No Hit
ATACGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA106270.20098041069827785No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC104640.19789771502275144No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG101960.19282923378937056No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG98480.18624777308333867No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT93290.1764323187545153No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA90940.17198794155360297No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA90160.17051278656776825No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC88930.16818658062856734No Hit
ATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAGACGTA87870.16618188282730473No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA85840.16234269741545282No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT82600.15621513055121625No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT79390.15014430041720409No Hit
GTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACC79220.14982279227926576No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA77320.1462294660317196No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA76400.14448953963817096No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT75790.1433358927902746No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA75730.14322241932982574No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA74360.14063144198291092No Hit
GGAGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGA73890.13974256654272843No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA70640.13359608743508372No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT69350.131156408035434No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTTAAGAA69130.13074033868045495No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA69130.13074033868045495No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA68500.12954886734574228No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA67670.12797915114286687No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA67080.12686332878178674No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA66870.12646617167021587No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT66440.12565294520366596No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA65200.12330782702105689No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC64910.12275937196222089No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG63950.1209437965950397No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA63290.11969558853010262No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA63210.11954429058283751No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG63090.11931734366193987No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT62470.11814478457063535No Hit
TTACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCT62050.11735047034749356No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT61930.11712352342659592No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA61720.11672636631502502No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA61050.11545924600667981No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA60170.1137949685867637No Hit
GATATGGACCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGG60150.11375714409994743No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG59690.1128871809031731No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC58990.11156332386460345No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT58650.11092030758872679No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA57600.10893452203087235No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA57450.10865083837975029No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT57290.1083482424852201No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT56160.10621115898010054No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA55280.10454688156018442No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG55110.1042253734222461No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT54870.1037714795804508No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT54680.10341214695569617No Hit
TGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGG54200.10250435927210558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA58750.0104.044572
TACGAAG20650.095.1588062
GGTACTG65000.094.5998465
ACGAAGC20900.094.021433
CAGGTAC66300.092.525343
AGCAGGT66700.091.42691
AGTAGAA78750.088.946811
GTACTGA70250.087.5301066
CGAAGCA24050.082.008284
AGCGAAA16500.081.7396551
ACTGATC75050.081.628938
CTGATCC80600.074.750199
TACTGAT82850.074.033517
GCGAAAG21050.064.070862
AGGTACT101550.062.835724
CGAACGC1051.6370905E-1162.14229145
TTTGGAC96150.060.7840423
TTGGACA96800.060.6755184
TTTTTGG95700.060.463711
ACAAGGC49150.060.3306358
Produced by FastQC (version 0.11.5)