FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1788_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1788_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4912560
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG76600.15592684873060075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG16850.033.9918259
GCTATAT12200.032.6884541
TATATAC18000.026.5871493
GTAACCG6050.026.367424
TATCGTC1658.423926E-526.3642027
TATACGG1409.9918E-425.8965765
GTCATAG23800.025.8963092
CATAGTG23400.025.409634
GTATATA22450.025.1924131
ATAGTGA26150.023.8466365
TAGTGAT27850.022.3910066
TATACCA41150.022.2024575
GCATCGA9200.022.06479145
AGTGATC32250.022.0314797
TATACTG19100.022.0188665
CGAAAGC43450.021.6947143
GTGATCC32300.021.5482318
ATATACC22600.020.533924
ATACCGC43450.020.1927726
ACTCCGC10850.020.0463098
Produced by FastQC (version 0.11.5)