FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1788_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1788_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863279
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG367060.7547582608359504No Hit
GTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAAT296880.6104523306189096No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC224370.4613553941692426No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA220790.45399410562297576No Hit
GTCTTCCGACTGGGCTCTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT216680.4455430173757253No Hit
GTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGGCGTTG208880.4295044557386076No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT208130.42796228635042327No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG202070.4155015576938934No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA193460.39779745311753656No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG174750.35932546744696325No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT161520.33212159943939057No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA150210.30886568506556994No Hit
GTCCTGTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTC149710.3078375721401137No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA147150.30257363396177767No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT146080.30037347230130124No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA142920.2938757986124177No Hit
CCCATATACAGCGCATCTGGATGCACCAGACGGCAGTCGCGCATGAAGAT138980.28577426875982237No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG136180.28001683637726726No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT130670.2686870319387393No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA129110.2654793196113157No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG125490.2580357820310124No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG122790.2524839722335486No Hit
CTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG116390.23932412678770845No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG112650.2316338421052956No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG108270.22262757287829876No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC107270.22057134702738626No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT106030.21802162697225472No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT104030.21390917527042969No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA103480.2127782510524278No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG103120.21203800974609927No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGA102750.21127720618126164No Hit
GGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT100710.20708250544540013No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA97830.20116057499477202No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGC96570.19856973042262227No Hit
AGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT96420.19826129654498542No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA94930.19519752002712573No Hit
GCATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCTCT94170.19363478838043222No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA93900.19307960740068583No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG93460.19217486802628433No Hit
ATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAC93320.19188699640715656No Hit
GGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCAT93000.19122900413486457No Hit
GATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATT89860.18477245496299924No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG89520.18407333817368898No Hit
GTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG88140.1812357464994297No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT87160.17922064516553543No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT86730.17833646804964304No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC84630.17401839376272674No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC83660.1720238546873416No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGTGCGGCTGCGGCGTGG83340.17136586241504959No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG83320.17132473789803135No Hit
CTCCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG81320.1672122861962063No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG80250.1650121245357299No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC80180.16486818872616604No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT80100.16470369065809304No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG78860.1621539706029615No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC78820.162071721568925No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG78760.16194834801787025No Hit
AAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGC78120.16063236347328624No Hit
CTTCAGGACCCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCG77190.15872007343193759No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAC77130.15859669988088285No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC73930.15201677715796277No Hit
GGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAACAC72530.14913806096668525No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG72490.14905581193264875No Hit
CCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGG72060.14817163481675635No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT71960.1479660122316651No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC71630.14728745770086396No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC70870.14572472605417044No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC68330.14050191239285265No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGC68070.1399672936716154No Hit
GTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGC68060.13994673141310626No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT67700.13920649010677777No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT67700.13920649010677777No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT67650.1391036788142321No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG65500.1346827932347702No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA65440.13455941968371546No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT65110.13388086515291434No Hit
CCCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGA64190.1319891373700748No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG64100.13180407704349267No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG63290.13013853410425352No Hit
ATCCTGATGTGTGCGGCTGCGGCGTGGGTCGGATGCTGGTGGAGCATGCG62780.12908985892028815No Hit
TTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCA62730.12898704762774252No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG61690.1268485727427935No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC61100.1256353994907551No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC59740.12283893233351406No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA59640.12263330974842282No Hit
AGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGC59450.12224262683674944No Hit
ATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCTCTTCGCTT58250.11977515581565441No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA58050.11936391064547192No Hit
GAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGA57950.11915828806038065No Hit
CTCCCTGACTCCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTG57370.11796567706685139No Hit
CTATAAGAAGGTGGGTTTTAAGGTTACGGGACGCTCTGAGGTGGACGATT56430.11603282476699361No Hit
ATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGGC56380.115930013474448No Hit
GGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATC55990.11512808539259213No Hit
CGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGG55400.11391491214055371No Hit
AGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGC54940.11296904824913397No Hit
CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGG54380.11181756177262296No Hit
GGTCAGAACAGGACGTGTTGATAAAACAGAATTTGCCTGGCGGCCGTAGC53950.11093338465673057No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT53890.11081001110567583No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG53400.1098024604387287No Hit
GCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGC53320.10963796237065569No Hit
TCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT53270.10953515107811006No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGG53110.10920615494196405No Hit
CCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCCTCA51570.10603956713155878No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT51390.10566944647839452No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC51370.10562832196137625No Hit
TAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAG50440.10371603192002761No Hit
CCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT50430.10369546966151849No Hit
GGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCA50130.10307860190624472No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTCAAGGAGTC50070.10295522835518998No Hit
GTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACT49740.10227667382438886No Hit
GATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCG49740.10227667382438886No Hit
GTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGA49510.10180374187867898No Hit
CCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTA48910.10057000636813146No Hit
ACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAC48870.10048775733409496No Hit
AGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGT48840.10042607055856759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTG39550.077.731264
TCATAGT39950.075.501043
GTCATAG41800.072.159482
CGAAAGC32650.070.396723
GGTCATA45750.066.880221
ATAGTGA49700.061.8565675
CGGTAGA11500.058.0051161
TAGTGAT54550.056.2251856
TGATCCG55250.055.2469449
GGACGGA23850.050.770726
AGTGATC61500.050.1050227
TAAGGAT18950.048.210064
GTTAAGG22100.046.9159052
AGGACGG27450.045.1679235
GTGATCC68250.045.041038
GACGGAT28550.042.410937
CGGTCCG1807.2759576E-1140.27719145
GCCTATA3700.039.188618145
TAAGACG5350.037.9472734
AAGGATC17150.035.5133365
Produced by FastQC (version 0.11.5)