FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1788_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1788_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4990034
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17750.048.610441
GCGAAAG18050.048.2042122
CGAAAGC24400.035.3621033
CCCTATC11500.028.3723722
ACTACCG4800.025.679885
CTAGTAG13000.025.0986373
ACCGTAC2051.2906183E-524.7585471
TATACCG1801.520348E-424.16935
TATACTG28700.023.748235
CCTATCC17300.021.3749473
CGCCTAT3406.43704E-821.3258516
TAGTACG20150.020.870766
CTACCGA8400.020.7165436
TACCGAT6300.020.7146767
TAGTACC8900.020.365969
GAGTACG7600.020.0336729
TCTAGTA17450.019.5291522
CGCTAAC1900.00588774619.0808331
TCTATAC44600.018.5332223
GTAGTAC22750.018.4855295
Produced by FastQC (version 0.11.5)