FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1788_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1788_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4547031
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC59080.12993093735230746No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG53620.11792310190979564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG14450.049.6779562
AGCGAAA14550.048.8376461
GGTACTG24750.040.1362655
CGAAAGC21850.032.8533863
GGATAGC26450.031.7997675
TGGATAG28200.029.5692524
CAGTATG25100.029.4618999
TACTGAT40750.026.8653937
ACTGATC41750.026.7422858
CAGGTAC38250.026.5395153
GTACTGA40300.026.4486716
AGGTACT39700.026.1178324
GCAGGTA40600.024.4675732
GTATGGA40300.024.2895971
TCTATAC34150.024.2053033
TTTGGAC36150.023.4678823
ATGCCGG9600.023.411145
CTATACC30700.023.146184
GACAGTA38850.022.3941237
TATAGCG3004.5563866E-721.7529222
Produced by FastQC (version 0.11.5)