FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1788_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1788_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454290
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5000.047.8485222
GATCCGT700.001056780941.428726
AGCGAAA6550.035.4187551
CGAAAGC7200.034.2350543
ACGTCCG2401.5979094E-624.1684139
CGCTATA1550.00180920723.3863752
TCTATAC14300.022.813983
TAGACCG1952.6154728E-422.3077725
TATAACG2303.143203E-522.0652945
CTATACC12300.021.219594
GTACCTA5550.020.9009746
GTCCTAT11550.020.0859621
TAACGTC2608.078176E-519.5199727
GTTCTAT14000.019.1601241
CACGAGC3802.080942E-719.07836145
TATACCA14600.018.869935
GTATTAA13350.017.9207951
GTGTAGG8750.017.3994641
GATATAC2550.001591792117.0582983
GAGCGAA4701.035296E-716.9675621
Produced by FastQC (version 0.11.5)