FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1788_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1788_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533394
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC80140.17677704607188344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG6850.050.803129
TCTATAC52300.030.918243
GTTCTAT54800.028.0521971
CTATACC57250.027.1050284
TTCTATA61150.026.2064022
CGAAAGC27500.024.7860153
TATACCA66200.023.5500645
AGCGAAA29250.023.3030931
TGCGACA8050.022.515121145
GCGAAAG31350.021.973422
TAGACCG2003.101824E-421.7536855
GTATAAG14350.021.728421
TACCACT67750.021.7235747
GAGTATA55700.020.565157145
ATACCAC72150.020.201156
CTAATAC18800.020.056593
TATACTG24600.020.0440465
TAAACCG3651.3603676E-719.8663795
ATAGACG3651.3603676E-719.8663793
TATACCG2359.1964385E-418.5137755
Produced by FastQC (version 0.11.5)