FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1787eye_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1787eye_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21107762
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC216800.10271103113631846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG46450.044.1719639
GCGAAAG73350.028.7674672
TATACTG129150.028.1852685
AGCGAAA76400.027.0495621
GTATTAT247300.023.6623691
TATACGG14150.022.5480585
CGAAAGC96700.021.521143
GTTGACA131100.020.8519861
GCACCGT114500.020.455546
GAGTGAT118600.019.9901147
AGTGATC118550.019.0805918
TCGCCAT58450.018.853354145
TTAGAGT148250.018.6843724
CCGACTA37250.018.492994
AGCACCG118900.018.4787965
GTATAAT106600.018.4340551
GTAATAT137150.018.2403031
ACTCGCG16350.017.736658145
TAATCGT11450.017.7295869
TAGAGTG153200.017.6074735
Produced by FastQC (version 0.11.5)