FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1787_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1787_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716200
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG10800.032.898262
GTGATCG4650.032.743049
AGCGAAA10500.031.0759071
CGAAAGC11650.028.0083273
GTATTAT27600.024.695591
AACCGCG1851.4677607E-723.5138727
ACCGTGC3753.092282E-1023.1999268
CCGTAGA2903.285786E-722.5032421
TATAATA21300.022.4680272
TATACTA6900.022.0683985
GTTGACA16400.021.6647071
TATACTG17100.021.6259235
GCACCGT17050.020.4134986
TATTATC15450.020.1809022
CTAATAC12050.019.8576323
TACACCG5900.019.663855
CTCCGAC3701.5726619E-719.59417145
CTATGCG6151.8189894E-1218.8645084
GTATATA14300.018.7614441
AGCACCG18650.018.6622075
Produced by FastQC (version 0.11.5)