FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1787_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1787_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4446745
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC44670.1004555017209217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT5200.041.831921
TATACTG26300.037.2196965
GTTGACA27750.032.1389771
AGCGAAA17600.029.6626381
GCTACTA8650.029.3354669
GTAATAT19350.028.8535771
TTACGTG8350.028.6561182
TAGCATG44500.027.699942
ACATGGT32750.027.6753755
GCGAAAG19550.027.4457362
CGAAAGC22150.025.2061713
TAGTGCA34950.022.8184729
GCGATTA4205.2750693E-1122.44013145
GTATTAT52350.022.2997041
CTACTGA42600.021.9570966
GGTAGTG35750.021.9024987
TCGACTG6350.021.6930689
GCAGTGC34550.021.406321
GTCGGCT6800.021.3263196
ATACTGT39650.021.2133676
Produced by FastQC (version 0.11.5)