FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1787_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1787_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349479
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT85870.13523944248024128No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC76960.12120679507720239No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT72830.11470232439543465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT7500.047.373431
ACCGTAC3050.045.1703761
TATACTG32800.038.4664575
GCTACTA10600.030.7790579
CCGTACA4450.029.330262
GTTGACA43350.028.4354291
TAGCATG56300.027.1754912
ACATGGT44100.027.1301485
AGCGAAA27000.026.5871261
TTACGTG12550.026.5777032
CCGACTA11850.026.3119754
GTAATAT27700.026.1770171
CTACTGA64750.024.9732656
GCGAAAG30200.024.0102372
ACGACTC5450.023.9487485
AACCGGC4850.023.916983145
GGTAGTG52300.022.874497
CGAAAGC32450.022.7923373
GTCGGCT9750.022.3114016
GCAGTGC42100.021.8736651
Produced by FastQC (version 0.11.5)