FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1787_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1787_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAAACTATTCCAAGGAAAATAAAATTAAAGGAAAGAAGTCGTAAAAG15.555555555555555No Hit
GGCGTCTATTTTTGTGGGTTAAACCTATCTATACAATCAATCTTACTATG15.555555555555555No Hit
GCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCTGCAGAGCAGAGAGCC15.555555555555555No Hit
GGCGTTCGCTCTGTCGCCTCAGGGAAGAAGAGCCTCGGGAGAGCTGGAGC15.555555555555555No Hit
AATATGCACATTATCATCTCTACTGGATGGCAGTCTCTAACTCTCAAATA15.555555555555555No Hit
CACAAATACAGATTCCTGAATCATCCATATCTACAGGATCAGAATTTCTG15.555555555555555No Hit
CACAAATAGATAGTTACACAACAATATTTGGTTACATATTTGATATTACA15.555555555555555No Hit
CTGAAGTGTTACTAACCATGTCTAAGGAGAATCAGCCTGTCCTTAATTTG15.555555555555555No Hit
GAGCTACTGTGGGTTGTCAAAACACTTCTTGGGTGGGTGGTTGGATGTGT15.555555555555555No Hit
CTTTCTCCCTCTGCCCCTCCCTCCTTTTATGTCTCCCTCTCTCTTTCAAA15.555555555555555No Hit
TACTTAGAGCGTTTACAGATGAAGGAGCAATAGCTGTCTCTTATACACAT15.555555555555555No Hit
CTTGTACTGCTTGGAGCCTAGCAGGCCCGGGGGAATAGGAGAGAGGTCAG15.555555555555555No Hit
AAAATAGAACTGTAAAATACCAAATTGAAAGCTTATGAATTGATCTATTT15.555555555555555No Hit
GAAGGACTTTACATGGTGATTGCCATCAGTCCTCAAAGACGTCTTTATAA15.555555555555555No Hit
TTATGAGAGAATATTTATCTCTTTTTGAATTTCCAAAGACTGTCAAATAG15.555555555555555No Hit
CATTTCTCCAAAAAGACATACAAATAGCTAACATACACACAAAAAAATGT15.555555555555555No Hit
AGCAAATAGATGCTTTGACTCAGAACTGGCTGTTTGACCAAGATATCAAT15.555555555555555No Hit
ACTGTAAATTCATAAACACAGAGACTAAACAACATTCTACTGAACAACCA15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)