FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9567476
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122012012.752788718780167No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC882450.9223435731639149Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG246650.25780048990977344No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATTAGATCGGAAGAGCGTCG198660.20764097030397566Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATAGATCGGAAGAGCGTCGT138610.14487624531276588Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTA131250.137183516321337Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAGATCGGAAGAGCGTCGTGT127420.13318037066411245Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGACTAGATCGGAAGAGCGTCG122640.1281842776506573Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAAGATCGGAAGAGCGTCGTG106460.11127281636243458Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105280.11003947122522179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA997200.067.6431352
ATAACTG1004700.067.300061
CCGTCCC111300.067.204413
GCCACCC176350.067.17705512
ACTGAAG979800.067.145844
CCCGTCC112650.066.7722552
CCCATAT695500.066.6030516
AGTCTGC185400.066.250577
GTCTGCC184050.066.2249458
GTGACTG278050.065.896611
TGACTGG277950.065.891822
AACTGAA1031700.065.840343
GCACACG157300.065.7788918
CGTCCCT114150.065.763144
CTGAAGA1045600.065.70965
GATAGTC310150.065.6752210
GAGTCTG188200.065.598346
GACTGGA280300.065.595853
ACCCATA492800.065.36349515
GGAGTTC246700.065.3590557
Produced by FastQC (version 0.11.5)