FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8822141
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1947392.2073893400706246Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1069011.2117353372611024No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC161750.18334551669487031Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC398300.043.98772449
CGCCGTA429900.043.9528547
TCGCCGT430750.043.70361746
CGTATCA404700.043.52559350
GGTCGCC474100.042.8448544
GTCGCCG459600.042.7116745
GCCGTAT453100.042.4911648
CTCGGTG388650.042.3610838
GTGGTCG478950.042.07604642
TGGTCGC478250.041.94736543
GATCTCG382400.041.727535
TCTCGGT392000.041.67882537
ATCTCGG394700.041.67747536
GTATCAT396050.041.3037751
GGTGGTC486850.041.19241
TCGGTGG461450.040.60781539
AGATCTC401000.040.04535734
CGGTGGT485000.040.0357740
TAGATCT432450.038.92981333
GAGTGTA515550.038.65559828