Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-9.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8822141 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 194739 | 2.2073893400706246 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 106901 | 1.2117353372611024 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 16175 | 0.18334551669487031 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTATC | 39830 | 0.0 | 43.987724 | 49 |
CGCCGTA | 42990 | 0.0 | 43.95285 | 47 |
TCGCCGT | 43075 | 0.0 | 43.703617 | 46 |
CGTATCA | 40470 | 0.0 | 43.525593 | 50 |
GGTCGCC | 47410 | 0.0 | 42.84485 | 44 |
GTCGCCG | 45960 | 0.0 | 42.71167 | 45 |
GCCGTAT | 45310 | 0.0 | 42.49116 | 48 |
CTCGGTG | 38865 | 0.0 | 42.36108 | 38 |
GTGGTCG | 47895 | 0.0 | 42.076046 | 42 |
TGGTCGC | 47825 | 0.0 | 41.947365 | 43 |
GATCTCG | 38240 | 0.0 | 41.7275 | 35 |
TCTCGGT | 39200 | 0.0 | 41.678825 | 37 |
ATCTCGG | 39470 | 0.0 | 41.677475 | 36 |
GTATCAT | 39605 | 0.0 | 41.30377 | 51 |
GGTGGTC | 48685 | 0.0 | 41.192 | 41 |
TCGGTGG | 46145 | 0.0 | 40.607815 | 39 |
AGATCTC | 40100 | 0.0 | 40.045357 | 34 |
CGGTGGT | 48500 | 0.0 | 40.03577 | 40 |
TAGATCT | 43245 | 0.0 | 38.929813 | 33 |
GAGTGTA | 51555 | 0.0 | 38.655598 | 28 |