Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10254110 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 271117 | 2.6439837294509227 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 127360 | 1.2420385581976399 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 21239 | 0.2071267033413919 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTATC | 54175 | 0.0 | 44.316784 | 49 |
CGCCGTA | 59480 | 0.0 | 44.153473 | 47 |
TCGCCGT | 59715 | 0.0 | 43.827328 | 46 |
CGTATCA | 55365 | 0.0 | 43.661133 | 50 |
GCACACG | 3105 | 0.0 | 43.058777 | 18 |
GGTCGCC | 65325 | 0.0 | 42.924427 | 44 |
GTCGCCG | 63645 | 0.0 | 42.880707 | 45 |
GCCGTAT | 62365 | 0.0 | 42.55988 | 48 |
GTGGTCG | 65565 | 0.0 | 42.4419 | 42 |
CTCGGTG | 54475 | 0.0 | 42.332485 | 38 |
TGGTCGC | 65545 | 0.0 | 42.139816 | 43 |
GTATCAT | 53835 | 0.0 | 41.976566 | 51 |
ATCTCGG | 55150 | 0.0 | 41.94169 | 36 |
TCTCGGT | 54735 | 0.0 | 41.792908 | 37 |
GATCTCG | 54015 | 0.0 | 41.79276 | 35 |
GGTGGTC | 67400 | 0.0 | 41.2137 | 41 |
TCGGTGG | 63920 | 0.0 | 40.851254 | 39 |
AGATCTC | 55890 | 0.0 | 40.57875 | 34 |
GTGACTG | 4980 | 0.0 | 39.689877 | 1 |
TAGATCT | 59530 | 0.0 | 39.66142 | 33 |