FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10254110
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2711172.6439837294509227Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1273601.2420385581976399No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC212390.2071267033413919Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC541750.044.31678449
CGCCGTA594800.044.15347347
TCGCCGT597150.043.82732846
CGTATCA553650.043.66113350
GCACACG31050.043.05877718
GGTCGCC653250.042.92442744
GTCGCCG636450.042.88070745
GCCGTAT623650.042.5598848
GTGGTCG655650.042.441942
CTCGGTG544750.042.33248538
TGGTCGC655450.042.13981643
GTATCAT538350.041.97656651
ATCTCGG551500.041.9416936
TCTCGGT547350.041.79290837
GATCTCG540150.041.7927635
GGTGGTC674000.041.213741
TCGGTGG639200.040.85125439
AGATCTC558900.040.5787534
GTGACTG49800.039.6898771
TAGATCT595300.039.6614233