Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7865481 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 109976 | 1.398210738796521 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 57731 | 0.7339792696721281 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 8327 | 0.10586765132354907 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCGTA | 25055 | 0.0 | 42.42292 | 47 |
TCGCCGT | 25290 | 0.0 | 41.807564 | 46 |
CCGTATC | 23655 | 0.0 | 41.58992 | 49 |
GGTCGCC | 27445 | 0.0 | 40.97325 | 44 |
GCACACG | 2005 | 0.0 | 40.8462 | 18 |
CGTATCA | 24285 | 0.0 | 40.798965 | 50 |
GTCGCCG | 26840 | 0.0 | 40.788445 | 45 |
CTCGGTG | 23530 | 0.0 | 40.517593 | 38 |
GCCGTAT | 26710 | 0.0 | 40.371117 | 48 |
GTGGTCG | 27835 | 0.0 | 40.22314 | 42 |
TCTCGGT | 23700 | 0.0 | 39.97693 | 37 |
ATCTCGG | 23925 | 0.0 | 39.95207 | 36 |
TGGTCGC | 27930 | 0.0 | 39.84849 | 43 |
GATCTCG | 23575 | 0.0 | 39.669273 | 35 |
GGTGGTC | 28525 | 0.0 | 39.029068 | 41 |
TCGGTGG | 27465 | 0.0 | 38.81508 | 39 |
GTATCAT | 24525 | 0.0 | 38.11644 | 51 |
AGATCTC | 25045 | 0.0 | 37.70474 | 34 |
CGGTGGT | 29160 | 0.0 | 37.567043 | 40 |
TAGATCT | 26770 | 0.0 | 36.412617 | 33 |