FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7865481
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1099761.398210738796521Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG577310.7339792696721281No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC83270.10586765132354907Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA250550.042.4229247
TCGCCGT252900.041.80756446
CCGTATC236550.041.5899249
GGTCGCC274450.040.9732544
GCACACG20050.040.846218
CGTATCA242850.040.79896550
GTCGCCG268400.040.78844545
CTCGGTG235300.040.51759338
GCCGTAT267100.040.37111748
GTGGTCG278350.040.2231442
TCTCGGT237000.039.9769337
ATCTCGG239250.039.9520736
TGGTCGC279300.039.8484943
GATCTCG235750.039.66927335
GGTGGTC285250.039.02906841
TCGGTGG274650.038.8150839
GTATCAT245250.038.1164451
AGATCTC250450.037.7047434
CGGTGGT291600.037.56704340
TAGATCT267700.036.41261733