Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8705013 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 237179 | 2.7246254543215502 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 137347 | 1.5777920147850442 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC | 10819 | 0.12428470813311823 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTATC | 46640 | 0.0 | 47.477077 | 49 |
CGTATCA | 48900 | 0.0 | 46.821342 | 50 |
CGCCGTA | 48215 | 0.0 | 46.804756 | 47 |
TCGCCGT | 47970 | 0.0 | 46.518505 | 46 |
GTATCAT | 48190 | 0.0 | 45.317642 | 51 |
GTCGCCG | 55425 | 0.0 | 45.092102 | 45 |
GGTCGCC | 55990 | 0.0 | 45.03713 | 44 |
GCCGTAT | 53475 | 0.0 | 44.825333 | 48 |
CTCGGTG | 53055 | 0.0 | 44.481457 | 38 |
GTGGTCG | 56270 | 0.0 | 44.44606 | 42 |
TGGTCGC | 54030 | 0.0 | 44.41696 | 43 |
ATCTCGG | 54380 | 0.0 | 44.138535 | 36 |
TCTCGGT | 52870 | 0.0 | 44.06856 | 37 |
GATCTCG | 53695 | 0.0 | 44.023727 | 35 |
GGTGGTC | 57290 | 0.0 | 43.556744 | 41 |
AGATCTC | 53640 | 0.0 | 43.40986 | 34 |
TCGGTGG | 60970 | 0.0 | 42.67279 | 39 |
CATTAAA | 56860 | 0.0 | 42.384037 | 55 |
TATCATT | 51990 | 0.0 | 42.341915 | 52 |
ATCATTA | 54420 | 0.0 | 42.33553 | 53 |