FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8705013
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2371792.7246254543215502Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1373471.5777920147850442No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC108190.12428470813311823Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC466400.047.47707749
CGTATCA489000.046.82134250
CGCCGTA482150.046.80475647
TCGCCGT479700.046.51850546
GTATCAT481900.045.31764251
GTCGCCG554250.045.09210245
GGTCGCC559900.045.0371344
GCCGTAT534750.044.82533348
CTCGGTG530550.044.48145738
GTGGTCG562700.044.4460642
TGGTCGC540300.044.4169643
ATCTCGG543800.044.13853536
TCTCGGT528700.044.0685637
GATCTCG536950.044.02372735
GGTGGTC572900.043.55674441
AGATCTC536400.043.4098634
TCGGTGG609700.042.6727939
CATTAAA568600.042.38403755
TATCATT519900.042.34191552
ATCATTA544200.042.3355353