Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-5.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8330960 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 321589 | 3.8601673756685906 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 123664 | 1.4843907544868777 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 25020 | 0.3003255327117163 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGACTGG | 5220 | 0.0 | 45.75537 | 2 |
GTGACTG | 5205 | 0.0 | 45.718605 | 1 |
GACTGGA | 5845 | 0.0 | 43.126137 | 3 |
CCGTATC | 69420 | 0.0 | 40.478413 | 49 |
CGTATCA | 70625 | 0.0 | 40.075188 | 50 |
CGCCGTA | 76705 | 0.0 | 39.882416 | 47 |
GTATCAT | 66345 | 0.0 | 39.658905 | 51 |
TCGCCGT | 76720 | 0.0 | 39.568977 | 46 |
GTCGCCG | 82345 | 0.0 | 38.646835 | 45 |
GCCGTAT | 80320 | 0.0 | 38.47544 | 48 |
CTCGGTG | 70540 | 0.0 | 38.46796 | 38 |
GGTCGCC | 85075 | 0.0 | 38.45547 | 44 |
GCACACG | 2110 | 0.0 | 38.316566 | 18 |
GGTGGTC | 84595 | 0.0 | 38.28524 | 41 |
TCTCGGT | 70270 | 0.0 | 38.207577 | 37 |
GTGGTCG | 85055 | 0.0 | 38.20186 | 42 |
ATCTCGG | 71145 | 0.0 | 38.155598 | 36 |
TGGTCGC | 84415 | 0.0 | 38.12664 | 43 |
GATCTCG | 69375 | 0.0 | 38.044403 | 35 |
CATTAAA | 74600 | 0.0 | 37.944508 | 55 |