FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8330960
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3215893.8601673756685906Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1236641.4843907544868777No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC250200.3003255327117163Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG52200.045.755372
GTGACTG52050.045.7186051
GACTGGA58450.043.1261373
CCGTATC694200.040.47841349
CGTATCA706250.040.07518850
CGCCGTA767050.039.88241647
GTATCAT663450.039.65890551
TCGCCGT767200.039.56897746
GTCGCCG823450.038.64683545
GCCGTAT803200.038.4754448
CTCGGTG705400.038.4679638
GGTCGCC850750.038.4554744
GCACACG21100.038.31656618
GGTGGTC845950.038.2852441
TCTCGGT702700.038.20757737
GTGGTCG850550.038.2018642
ATCTCGG711450.038.15559836
TGGTCGC844150.038.1266443
GATCTCG693750.038.04440335
CATTAAA746000.037.94450855