Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9082613 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 235423 | 2.5920183982296727 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 93638 | 1.0309588220922767 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 18403 | 0.20261790301975874 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTATC | 46790 | 0.0 | 43.58456 | 49 |
CGCCGTA | 51030 | 0.0 | 43.248302 | 47 |
CGTATCA | 47655 | 0.0 | 43.094543 | 50 |
TCGCCGT | 51080 | 0.0 | 42.99381 | 46 |
GTCGCCG | 54165 | 0.0 | 42.21257 | 45 |
GGTCGCC | 55985 | 0.0 | 42.065567 | 44 |
GCCGTAT | 53640 | 0.0 | 41.7901 | 48 |
CTCGGTG | 46690 | 0.0 | 41.60272 | 38 |
TGGTCGC | 55985 | 0.0 | 41.484642 | 43 |
GTATCAT | 46265 | 0.0 | 41.431976 | 51 |
GTGGTCG | 56610 | 0.0 | 41.366665 | 42 |
TCTCGGT | 46720 | 0.0 | 41.141747 | 37 |
ATCTCGG | 47135 | 0.0 | 41.0988 | 36 |
GATCTCG | 46195 | 0.0 | 40.889565 | 35 |
GGTGGTC | 57170 | 0.0 | 40.692104 | 41 |
TCGGTGG | 54465 | 0.0 | 40.238873 | 39 |
AGATCTC | 48105 | 0.0 | 39.550667 | 34 |
CGGTGGT | 57160 | 0.0 | 39.425648 | 40 |
TAGATCT | 51055 | 0.0 | 38.999786 | 33 |
CATTAAA | 53245 | 0.0 | 38.577206 | 55 |