FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9082613
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2354232.5920183982296727Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG936381.0309588220922767No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC184030.20261790301975874Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC467900.043.5845649
CGCCGTA510300.043.24830247
CGTATCA476550.043.09454350
TCGCCGT510800.042.9938146
GTCGCCG541650.042.2125745
GGTCGCC559850.042.06556744
GCCGTAT536400.041.790148
CTCGGTG466900.041.6027238
TGGTCGC559850.041.48464243
GTATCAT462650.041.43197651
GTGGTCG566100.041.36666542
TCTCGGT467200.041.14174737
ATCTCGG471350.041.098836
GATCTCG461950.040.88956535
GGTGGTC571700.040.69210441
TCGGTGG544650.040.23887339
AGATCTC481050.039.55066734
CGGTGGT571600.039.42564840
TAGATCT510550.038.99978633
CATTAAA532450.038.57720655