FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414113
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1288992.009615359130717Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG742261.1572293784035297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA251500.048.2736747
TCGCCGT252350.047.54244646
CCGTATC248750.047.41483349
CGTATCA257950.046.74133350
GGTCGCC293550.046.06796644
GTCGCCG289400.045.9304245
TGGTCGC282900.045.5757743
GCCGTAT278850.045.434148
CTCGGTG278500.045.1278238
GTGGTCG295750.045.0392842
ATCTCGG284450.044.9843236
GATCTCG281550.044.71423735
TCTCGGT278350.044.58701737
GTATCAT259550.044.06648651
GGTGGTC302500.043.82601541
AGATCTC285000.043.35050634
TCGGTGG322500.042.80106439
TAGATCT299050.042.2852133
CGGTGGT330300.041.58940
CATTAAA317100.040.33992855
Produced by FastQC (version 0.11.5)