FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998633
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1579001.754710965543322Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG859270.9548894815468082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC340750.043.04577649
CGCCGTA353500.042.88894747
CGTATCA355100.042.21299450
TCGCCGT354300.042.15002446
GCCGTAT387850.040.77790548
GGTCGCC407950.040.4328644
GTCGCCG403650.040.2915645
GTATCAT352750.040.07334551
GTGACTG39650.040.0401231
GTGGTCG409200.039.7878542
CTCGGTG382650.039.6694138
TGGTCGC399850.039.5891243
ATCTCGG392800.039.35783836
GCACACG22400.039.0605518
TCTCGGT384200.039.0545337
GATCTCG389650.038.97537235
AGATCTC395650.038.0397334
GGTGGTC429050.037.94706341
TCGGTGG441550.037.88736339
TAGATCT419650.036.8233833