FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8885968
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1382391.5556999530045574Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG764660.8605252686032631No Hit
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA115220.12966510795447383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA294600.044.56147847
TCGCCGT294900.043.98209446
CCGTATC280600.043.91657649
GGTCGCC328200.043.48644644
GTCGCCG320350.043.35030745
CGTATCA290900.042.75863650
GTGGTCG333450.042.2772242
GCACACG20650.042.20236218
GCCGTAT317250.042.01985548
CTCGGTG291600.041.99633838
TGGTCGC335150.041.6868443
TCTCGGT291750.041.53205537
ATCTCGG297400.041.39007636
GATCTCG291500.041.3513235
GGTGGTC345450.040.81875641
TCGGTGG337600.040.5653439
AGATCTC303200.039.8255734
CGGTGGT355700.039.26861240
GTATCAT298250.039.264151
GTAGGGA389950.038.5219519