Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-20.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8885968 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 138239 | 1.5556999530045574 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 76466 | 0.8605252686032631 | No Hit |
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA | 11522 | 0.12966510795447383 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCGTA | 29460 | 0.0 | 44.561478 | 47 |
TCGCCGT | 29490 | 0.0 | 43.982094 | 46 |
CCGTATC | 28060 | 0.0 | 43.916576 | 49 |
GGTCGCC | 32820 | 0.0 | 43.486446 | 44 |
GTCGCCG | 32035 | 0.0 | 43.350307 | 45 |
CGTATCA | 29090 | 0.0 | 42.758636 | 50 |
GTGGTCG | 33345 | 0.0 | 42.27722 | 42 |
GCACACG | 2065 | 0.0 | 42.202362 | 18 |
GCCGTAT | 31725 | 0.0 | 42.019855 | 48 |
CTCGGTG | 29160 | 0.0 | 41.996338 | 38 |
TGGTCGC | 33515 | 0.0 | 41.68684 | 43 |
TCTCGGT | 29175 | 0.0 | 41.532055 | 37 |
ATCTCGG | 29740 | 0.0 | 41.390076 | 36 |
GATCTCG | 29150 | 0.0 | 41.35132 | 35 |
GGTGGTC | 34545 | 0.0 | 40.818756 | 41 |
TCGGTGG | 33760 | 0.0 | 40.56534 | 39 |
AGATCTC | 30320 | 0.0 | 39.82557 | 34 |
CGGTGGT | 35570 | 0.0 | 39.268612 | 40 |
GTATCAT | 29825 | 0.0 | 39.2641 | 51 |
GTAGGGA | 38995 | 0.0 | 38.52195 | 19 |